Figure 2
From: Proximal GATA-binding sites are essential for human HSD3B1 gene transcription in the placenta
Enhancement of the transcriptional activity of the HSD3B1 promoter by two putative GATA sites. (A) Schematic of the promoter region in the HSD3B1 gene. “+1” is the transcription start site indicated by the arrow. Two putative GATA-binding sites are indicated as Gu and Gp. Their sequences are shown with mutated nucleotides in lowercase letters. E1, exon 1; I1, intron 1; E2, exon 2. (B) JEG-3 cells were transiently transfected with the −238/+337 HSD3B1 promoter-luciferase construct containing wild-type or mutated GATA sites (either or both sites). Cells were harvested after 24 h of transfection and assayed for luciferase activity. Results are presented as the activity relative to the wild-type construct. Data are means ± SD of five independent experiments. ***P < 0.001 compared with wild-type construct. # P < 0.05.
