Figure 3
From: Proximal GATA-binding sites are essential for human HSD3B1 gene transcription in the placenta
Effects of GATA factors on the transcriptional activity of the HSD3B1 promoter. (A) HSD3B1 promoter-luciferase constructs containing wild-type or mutated GATA sites were co-transfected into HeLa cells with the expression vector for GATA2, GATA3, GATA4 or empty vector control (v) as indicated in (A,C,D), respectively. Cells were harvested after 24 h of transfection and assayed for luciferase activity. Results are presented as the activity relative to the empty vector. Data are means ± SD of nine (A), four (C) or five (D) independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the vector control. (B) Expressions of GATA proteins were detected by western blot analysis with anti-GATA3 and anti-Myc for GATA2 and GATA4. N, non-transfected cells; V, vector control. Uncropped images of blots were shown in Supplementary Fig. S3.
