Figure 6

Curdlan induces MMP7 in an mTOR-independent manner. MMP-7 secretion was measured by immunoblotting (A,C,E) or ELISA (B,D,F) in the cell supernatant of unstimulated or Curdlan-stimulated cells. Cells were pre-incubated with 30 μM PD98059, 20 μM SP600125, 10 μM SR11302, 10 μM Bay 11-7085 and (100 nM) Rapamycin prior to Curdlan stimulation as indicated. (G) Phosphorylation of mTOR, S6K and ERK were detected by immunoblotting in the cell lysate of Curdlan-stimulated cells in the presence of 30 μM PD98059 and (100 nM) Rapamycin. Total mTOR, S6K and ERK were used as controls. Data are representative of at least three independent experiments. *p < 0.05, **p < 0.01, ns, not significant (p > 0.05).