Figure 7

CpG and Curdlan have synergistic effect on MMP-7. MMP-7 was detected by Immunoblotting (A) or ELISA (B,C) in the supernatant of B-lymphocytes after stimulation with CpG, Curdlan and the combination of CpG and Curdlan overnight. Cells were pre-incubated with 30 μM PD98059 and the 20 μM SP600125, 10 μM SR11302, 10 μM Bay 117085 and (100 nM) Rapamycin prior to Curdlan stimulation as indicated. Data are representative of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.0001.