Figure 6 | Scientific Reports

Figure 6

From: Genome-wide identification of Major Intrinsic Proteins in Glycine soja and characterization of GmTIP2;1 function under salt and water stress

Figure 6

Overexpression of GmTIP2;1, GmTIP1;7 and GmTIP1;8 improved salt and drought tolerance in Saccharomyces cerevisiae and soybean. (a) Various combination of overexpressing yeast cells were plated on 30% PEG 6000 and 5 M NaCl into SC-Ura medium. According to the survival patterns of the yeast cells, it is visible that overexpression of GmTIP2;1 improved salt and drought tolerance in Saccharomyces cerevisiae. (b,c) Growth curves depict analysis of the growth of yeast strains overexpressing the indicated proteins in YPDA medium supplemented with NaCl (0.5 M) and PEG (10%). X-axis shows time and Y-axis shows OD600 values. OD were measured every 2 h. (d) Phenotypic analysis of the hairy root transformed transgenic plants treated by 200 mM NaCl solution for seven days. The photograph was documented after 7 days of growth of these respective seedlings in hydroponic media containing 200 mM NaCl. (e) Real-time RT-PCR analysis of GmTIP2;1, GmTIP1;7 and GmTIP1;8 under drought stress: cultivated soybean seedlings were treated with 30% PEG 6000 for 0, 2, 4 and 8 h (P0–8) and relative expression of GmTIP2;1, GmTIP1;7 and GmTIP1;8 were plotted compared to control.

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