Figure 3
From: Biomimetic Molecular Signaling using DNA Walkers on Microparticles
Demonstration of OSD reaction on particle surface. (a) Schematic shows how sensor particles are prepared. Cholesterol DNA is displaced by the quenched detector complex (TR-QOSD). (b) A schematic shows how the presence of TR-QOSD was verified. A specific ssDNA displaces the quencher molecule (QOSD). This results in increased Texas Red fluorescence on the microparticle surface. (c) Fluorescence micrograph shows initial green fluorescence of the sensor microparticles. (d) Fluorescence micrograph shows the increased red fluorescence after addition of ssDNA. (e) Calibration curve shows the mean fluorescence and standard deviation among several images at various concentrations of ssDNA.
