Figure 1
From: The structure-function relationship of disulfide bonds in etanercept
Potency loss in late eluting large scale HIC fractions. Large scale HIC separation of GP2015 (Erelzi®) resulted in the separation of a main peak from a late eluting shoulder peak. Assessment of potency in these two fractions revealed near 100% in the main peak while the late eluting fraction was found to be inactive. Although high and low molecular weight variants were slightly enriched in the late eluting fraction, this moderate decrease in purity could not explain the loss of potency.
