Figure 3
From: The structure-function relationship of disulfide bonds in etanercept
Approximation of overall incorrect disulfide bridging using analytical hydrophobic interaction chromatography. Samples with different potencies, such as GP2015 (Erelzi®) drug substance, process intermediates and late eluting HIC fractions were analyzed using the analytical HIC and the non reducing peptide map methods. The abundance of incorrect disulfide bridging assessed by these methods was found to correlate, allowing approximation of how the relative amount of C78-C88 assessed by the non reducing peptide map corresponds with overall incorrect disulfide bridging in etanercept, as reflected by the HIC post peak.
