Figure 4
From: The structure-function relationship of disulfide bonds in etanercept
Correlation of incorrect disulfide bridge C78-C88 and potency. The relative amount of C78-C88 was quantified by non reducing peptide map analysis in different samples including reference product (Enbrel®), GP2015 (Erelzi®), process intermediates and late eluting HIC fractions. Evaluation of incorrect disulfide bridging relative to potency in these samples revealed a strong inverse correlation between potency and the relative abundance of C78-C88.
