Figure 2
From: Wnt2b attenuates HSCs activation and liver fibrosis through negative regulating TLR4 signaling
Wnt2b is mainly produced by hepatocytes during liver fibrogenesis. (a) mRNA and protein levels of Wnt2b in hepatocytes (left) and NPCs (right) from control and TAA-treated mice, respectively. (b) mRNA (upper) and protein (lower) levels of Wnt2b in primary mouse hepatocytes treated with CCl4 in vitro. (c) Immunofluorescence staining of Wnt2b in HSCs from control and TAA-treated mice. (d) RT-PCR analysis of Wnt2b in cultured HSCs from naive mice at the indicated points of time. Mouse embryonic tissues served as positive controls. Cropped blots are displayed; Full-length blots are presented in Supplementary Fig. S5. Data are mean ± SEM of three independent experiments; *P < 0.05, **P < 0.01, ***P < 0.001.
