Figure 2

Effects of RNAi silencing of target genes on five consecutive days at different concentrations. Effect of orally administered dsRNAs against target genes (boul, zpg, dsx M, fzo and gas8) (A–E) at different concentrations (250, 500, 1000 & 2000 ng/ul) were analyzed. Normalized target gene expression is reported relative to the expression of the ds-EGFP control, which was set to 1-fold. All error bars depict the SE of the mean of three independent replicates. Treatments were compared with their respective controls using ANOVA (Dunnett-test, P < 0.05). *, **, *** and ns indicates P < 0.05, P < 0.01, P < 0.001 and non-significant, respectively.