Table 2 Primer sets for quantitative RT-PCR analysis.
From: Herbal medicine Yinchenhaotang protects against α-naphthylisothiocyanate-induced cholestasis in rats
Gene | Full name | ID | Sequences (Forward/Reverse 5′-3′) | Amplicon Size (bp) |
|---|---|---|---|---|
IL-10 | Interleukin-10 | 25325 | AGTGGAGCAGGTGAAGAATGA-F | 21 |
CACGTAGGCTTCTATGCAGTTG-R | 22 | |||
IL-6 | Interleukin-6 | 24498 | AGTTGCCTTCTTGGGACTGA-F | 20 |
ACTGGTCTGTTGTGGGTGGT-R | 20 | |||
TGF-β1 | Transforming growth factor β1 | 59086 | ATTCCTGGCGTTACCTTGG-F | 19 |
AGCCCTGTATTCCGTCTCCT-R | 20 | |||
IL-17A | Interleukin-17 A | 301289 | ACAAGCTCATCCCGTACCAG-F | 20 |
AGCCTCCAGGTTCAGTAGCA-R | 20 | |||
IL-17F | Interleukin-17 F | 301291 | CGGATGGAGTAGAAGCAGGA-F | 20 |
CAGCAGCAGAGACTTGACCA-R | 20 | |||
α-SMA | α-smooth muscle actin | 81633 | AGGGAGTGATGGTTGGAATG-F | 20 |
GGTGATGATGCCGTGTTCTA-R | 20 | |||
FXR | Farnesoid X receptor | 60351 | AAGTGACCTCCACGACCAAG-F | 20 |
TGGCATTCTCTGTTTGCTGT-R | 20 | |||
ACTB | β-actin | 81822 | TGACGAGGCCCAGAGCAAGA-F | 20 |
ATGGGCACAGTGTGGGTGAC-R | 20 | |||
CAR | Constitutive androstane receptor | 65035 | GCATTGGATTGGAAAGGGTAA-F | 21 |
ACT GAT GAC CGC ACG AAG A-R | 19 | |||
VDR | Vitamin D receptor | 24873 | GGATTCTGATGACCCGTCTG-F | 20 |
CGATGACCTTTTGGATGCTG-R | 22 | |||
TGR5 | The G-protein-coupled bile acid receptor | 388443 | AAGGTGGCTACAAGTGCT TC-F | 20 |
ATTGGCTACTGGAGTGGTAGG-R | 21 | |||
NTCP | Na+/taurocholate Cotransporting Polypeptide | 24777 | CCACTTTTCTTCTTTCCTCTCCTCT-F | 25 |
GGTCCTTTGGAGGCTTGATTT-R | 21 | |||
ASBT | Apical sodium-dependent bile acid transporter | 29500 | GGTACAGGTGCCGAACAGTAG-F | 21 |
AGATGAGTGGGAAGGTGAACA-R | 21 | |||
BSEP | Bile salt export pump | 83569 | TGGTTTCAAGGCAATGTTAGG-F | 21 |
TGGGAAGCATCTGTAGCAAG-R | 20 | |||
OATP1a1 | Organic anion transporter | 50572 | AGGTTATCCATAAGGCCGTCAAAG-F | 24 |
TTAAGGTTAAGGGTGAAGGCCACTA-R | 25 | |||
MRP2 | Multi-drug related protein-2 | 25303 | CTGGAGTTGGCTCACCTCA-F | 19 |
CACCCTCTGTCACTTCGGATA-R | 21 | |||
MRP3 | Multi-drug related protein-3 | 140668 | GCAGTGATCGGGAGAAACA-F | 19 |
AAGGCATAGGACACGGAAAGA-R | 21 | |||
MRP4 | Multi-drug related protein-4 | 170924 | CCTTGCGAGGGCAATTCTG-F | 19 |
CTTGTCGCTGTCAATGATGGTG-R | 22 |
