Figure 1 | Scientific Reports

Figure 1

From: PARP-1 overexpression contributes to Cadmium-induced death in rat proximal tubular cells via parthanatos and the MAPK signalling pathway

Figure 1

Effects of cadmium on cell proliferation and survival. (A) Cd inhibited the proliferation of NRK-52E cells. Normalized cell index were determined by RTCA after treated with 1.25, 2.5, 5 or 10 μM Cd and Error bars indicate SD (n = 3). (B) Western blot analysis of the expression of ERK1/2 and p-ERK1/2 protein in NRK-52E cells after treated with 1.25, 2.5, 5 or 10 μM Cd for 12 h, and the rate of p-ERK1/2/ERK1/2 was significantly increased in 5, 10 μM Cd group that compared with the control group. (C) Cell counting kit-8 method to detect the effect of Cd (1.25, 2.5, 5 μM) on the proliferation of primary rPT cells, and compared with the control group, the survival rate of primary rPT cells decreased gradually. (D) Western blot analysis of the expression of ERK1/2 and p-ERK1/2 protein in primary rPT cells after treated with 1.25, 2.5 or 5 μM Cd for 12 h, and the rate of p-ERK1/2/ERK1/2 was significantly increased in 2.5, 5 μM Cd group that compared with the control group. Semi-quantitative analysis was performed with images from three independent experiments (mean ± SD, n = 3). *P < 0.05; **P < 0.01 vs. the control group by one-way ANOVA.

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