Figure 7

The effect of PARP-1 protein on mitochondrial membrane potential and proliferation of rat proximal tubular cells. (A and B) Flow cytometry analysis combined with JC-1 staining showed the decline in the mitochondrial membrane potential caused by Cd treatment for 12 hours in rat renal tubular epithelial cells, which was inhibited by PARP-1 gene knockout or PARP-1 inhibitor DPQ at 25 μM. (C) Cell index for NRK-52E cells treated with 5 μM Cd and 25 μM DPQ as indicated. Results were normalized to the time of treatment and error bars indicate SD (n = 4).