Figure 6

Reduced inflammatory cell infiltration in IGF1R-deficient lungs after BLM treatment. (a) Lung mRNA expression of neutrophil chemotaxis (Cxcl1), neutrophil (Ly6g) and macrophage (Adgre1 and Marco) markers and (b) representative H&E, as well as F4/80 and CD3 immunostained sections, and quantification of neutrophils (red arrowheads, upper panels), alveolar macrophages (white arrowheads, middle panels) and lymphocytes (orange arrowheads, bottom panels) in BLM treated UBC-CreERT2; Igf1r ^fl/fl (CreERT2) vs. Igf1r ^fl/fl lungs at D3. Scale bars: 10 μm (upper panels) and 20 μm (middle and bottom panels). Numbers within graphic bars indicate the number of mice analyzed and data are expressed as mean ± SEM. *p < 0.05; **p < 0.01 (Mann-Whitney U test).