Figure 3

Label- retaining cells (LRCs) detected by BrdU immunofluorescent staining. (A) A number of slow cycling LRCs were detected in limbal (top panel), peripheral (middle panel) and central cornea (bottom panel) in C57BL/6 mice with 4 weeks chasing after BrdU injections. See method for detail. (B) Double staining of LRCs with epithelial cell marker K14 and stem cell marker NGF. Image magnification: 400×.