Figure 1 | Scientific Reports

Figure 1

From: Structural alteration of a BYDV-like translation element (BTE) that attenuates p35 expression in three mild Tobacco bushy top virus isolates

Figure 1

The effect of the BTE on translation of the fire luciferase reporter gene or p35 in TBTV. (A) Genome organization of the TBTV. RdRp: RNA-dependent RNA polymerase, BTE: Barley yellow dwarf virus (BYDV)-like translation elements, fs: frameshift. (B) Secondary structure of the BTE and putative RNA-RNA interactions with the 5′ UTR in TBTV. Bold nucleotides indicating the 17 nt consensus sequences of the BTE and the loop sequences that base-pair with the 5′ UTR; the broken polyline indicates potential base pairing, SL: stem-loop. (C) The effect of the BTE on Fluc translation. WGE: wheat germ extract. (D), (E) and (F) The effect of the BTE on p35 translation in full-length TBTV. wt: wild type TBTV (TBTV-ch),ck−: mock, ck+: purified p35, M: western blotting marker. The numbers under the western blots indicate the relative p35 level, and the numbers in bracket indicate the STEV.

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