Figure 6 | Scientific Reports

Figure 6

From: Structural alteration of a BYDV-like translation element (BTE) that attenuates p35 expression in three mild Tobacco bushy top virus isolates

Figure 6

Structure probing of the BTE region in different TBTV isolates and chimeric TBTV using SHAPE. (A) SHAPE structure probing of the BTE region in TBTV-Ch and three new TBTV isolates. D:DMSO; wt:TBTV-Ch; JC:TBTV-JC; MD-I:TBTV-MD-I; MD-II:TBTV-MD-II. Red triangle: moderately high to high reactivity to NMIA compared with the DMSO control; green triangle: low to moderate reactivity to NMIA compared with the DMSO control. Black solid circle: higher NMIA activity in the new TBTV isolates compared with the wt; black empty circle: lower NMIA activity in the new TBTV isolates compared with the wt. (B) SHAPE structure probing of the BTE region in TBTV-Ch and chimeric TBTV with different RV regions. Red solid circle: higher NMIA activity in chimeric TBTV compared with the wt; red hollow circle: lower NMIA activity in chimeric TBTV compared with the wt.

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