Figure 1
Domain 3 (ID3) of CBP is fully disordered. (a) CBP domain organization. CBP globular domains (colored; Br: Bromodomain, R: Ring) are connected by intrinsically disordered regions (IDRs, grey) of different lengths. The intrinsically disordered region 3 (ID3, aa674–1080) is located between the KIX domain and bromodomain of CBP. (b) Neighbor corrected structural propensities (ncSP) obtained from experimentally measured N, C′, Cα and Cβ chemical shifts. Positive and negative values correspond to α-helical and β-sheet propensities, respectively. (c) SAXS Kratky plot also shows an overall disordered state, with a minor tendency for local secondary structure formation. (d) Ensemble of selected conformations ID3 may adopt in solution. (e) Rg distributions of a random pool of ID3 conformations (teal), of the pool of ID3 conformations biased by experimental ncSP scores (grey) and the ensemble calculated by Flexible Meccano and selected by GAJOE (green).
