Figure 6
CTR of ID3 specifically interacts with the middle region of ZFP106-f. (a) Representation of the ZFP106 fragment identified in the Y2H assay (blue) and the three positions (M1, M2 and M3) where a paramagnetic label was attached to the protein. (b) I (para) /I(dia) plots of the 2D 1H-15N HSQC cross-peaks intensities of ID3 in the presence of the paramagnetically tagged single-Cys ZFP106-f mutants M1, M2 and M3 showing the site of interaction within ID3 (red box). A control experiment with an unrelated IDP, domain 1 of human Calpastatin (hCSD1), labeled with the same paramagnetic probe, is also shown to outline sticky region(s) of ID3 engaged in non-specific interaction (blue box). (c) Charge distribution plots of ID3 and ZFP106-f amino acid sequences calculated with the EMBOSS website (http://www.bioinformatics.nl/cgi-bin/emboss/charge). The regions involved in the interaction are highlighted (red): the middle region of ZFP106-f close to the M2 site, and the C-terminal 100 residues (CTR) of ID3.
