Figure 4

Clonogenic analysis in NSMC medium. HSR-GBM1 (A,B), HSR040622 (C,D), and HSR040821 (EF) were treated for 72 hours with ACF (5 μM) or vehicle (DMSO) in normoxia (A,C,E) or hypoxia (B,D,F). Following 24 hours of recovery (in normoxia and without drug), cells were plated in NSMC medium. The clonogenic potential of ACF-treated GSCs was significantly lower as compared with vehicle in each of three GSC lines. (****p < 0.0001, One way ANOVA with multiple comparisons).