Figure 2

(A,B) Peripheral blood mononuclear cells (PBMCs) were isolated from healthy controls (A and B) and pre-treated with ethanol ranging from 6.25 mM–50 mM (1.4 mg–10 mg%) followed by treatment with LPS (10 ng/ml for 6 hrs). Ethanol primes PBMCs for LPS-induced inflammatory responses, as indicated by the increase in the levels of sCD14 and sCD163 in the supernatant in the dose dependent manner. (C,D) PBMCs were isolated from excessive drinkers and subjected to the stimulation with LPS (10 ng/ml for 6 hrs). sCD14 and sCD163 were significantly increased in supernatants of LPS stimulated PMBCs from subjects with EAU compared to controls. *Compared to controls and ^compared to LPS treatment alone, p < 0.05.