Figure 4
From: Attenuation of Innate Immunity by Andrographolide Derivatives Through NF-κB Signaling Pathway
Treatment with andrographolide analogs inhibited the NF-κB signaling pathway. AD-293 cells were treated with 50 ng/ml TNF-α alone or in combination with either (A) 3b and 5a, or (B) 3a, 5b, and 6b for 3 h. After fixation, the nuclear translocation of NF-κB p65 was examined under a confocal microscope. Images were obtained under 200× magnificence. (C) Western blot analysis of the levels of phosphorylated p65, p65, phosphorylated IκBα, and IκBα treated with 1 µg/ml LPS alone or in combination with compounds 3b, 5a, 3a, 5b, and 6b in THP-1 cells. The blot of actin was used as an internal loading control. The results are representative of three independent experiments.
