Table 2 List of 59 candidate metabolites affected by CTB expression in MR-CTB51A.

From: Seed Metabolome Analysis of a Transgenic Rice Line Expressing Cholera Toxin B-subunit

Categorya

Metaboliteb

Fold differencec

MR-CTB51A/NPB-HP

MR-CTB51A/MR-RNAi

Amino acids

l-Aspartic acid-like 3

12.17

2.37

Ornithine

9.57

7.10

l-Lysine

8.88

5.22

l-Glutamine

8.18

32.70

l-Phenylalanine-like 1

5.59

4.29

l-Valine

4.58

3.37

Glycine

4.16

1.73

l-Leucine

3.63

2.49

l-Isoleucine

3.27

1.99

Amines

Putrescine

6.72

9.25

Organic acids

l-Malic acid-like 5

14.00

11.70

Oxoglutaric acid

12.92

3.91

Carbohydrates

d-Ribose-like 3

6.33

18.13

Mannitol-like 1

2.61

92.55

Lipids

Fatty acid methyl esters

C15:0 ME

3.46

3.16

C23:0 ME

3.26

2.69

C21:0 ME

2.68

2.29

C16:1(9Z) ME-like 1

2.40

1.80

C20:1(11Z) ME*†

2.35

2.06

C22:0 ME

2.34

1.95

C22:1(13Z) ME-like 1

2.25

1.70

C17:0 ME

2.17

2.48

C20:0 ME

2.16

1.72

C18:0 ME

1.98

1.68

C14:0 ME

1.80

1.79

C16:0 ME-like 1

1.77

1.63

C18:1 ME

1.71

1.64

C18:1 ME-like 1†

1.66

1.59

C18:2 ME

1.54

1.60

Fatty acids and conjugates

C19:0-like 1

6.94

4.47

C23:0-like 1

5.49

2.57

C22:0

4.15

2.09

C20:0

3.68

1.78

C17:0

3.64

2.43

C15:0

3.45

2.61

C14:0

2.24

1.78

C18:0-like 1

2.20

1.56

C17:0-like 3†

2.15

2.07

C21:0-like 1

2.14

2.43

C16:0-like 1

1.96

1.56

C13:0-like 1

1.73

1.66

C18:2-like 1

1.69

1.66

Hydrocarbons

Undecane-like 2

1.79

2.26

Heptadecane-like 1†

1.64

1.76

Eicosane†

1.60

1.59

Pentadecane†

1.44

1.63

Sterols

Cycloartenol

11.09

3.87

24-Methylenecycloartanol

8.70

6.31

Stigmasterol

3.85

3.14

Cholesterol

3.84

2.77

Campesterol

3.71

2.73

Brassicasterol-like 1

2.86

2.65

β-Sitosterol

2.77

2.44

Prenol lipids

β-Tocopherol

3.04

5.15

Squalene

2.71

2.86

α-Tocopherol

1.72

2.52

Others

1-Kestose

67.90

95.14

l-3-Cyanoalanine

10.58

13.48

Aminoadipic acid

4.62

5.74

  1. a)Categories were assigned as in Table 1. b)Metabolites showing significant differences in relative levels between MR-CTB51A and NPB-HP (Table 1) and between MR-CTB51A and MR-RNAi (Supplementary Table S8) were selected. ME, methyl ester. *Metabolites showing significant difference in relative levels between NPB-HP and NPB-PF (Supplementary Table S6). †Metabolites showing no significant difference in relative levels between MR-CTB51A and NPB-PF (Supplementary Table S7). c) Bold numbers, fold difference >5. MR-RNAi, MucoRice-RNAi; the names of other lines are explained in Table 1.
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