Figure 3 | Scientific Reports

Figure 3

From: Chronic stress and intestinal barrier dysfunction: Glucocorticoid receptor and transcription repressor HES1 regulate tight junction protein Claudin-1 promoter

Figure 3

Realtime PCR analysis of mRNA levels in rat colon luminal epithelium from the following groups: control (CT), water avoidance (WA) stress, WA stress with NR3C1 antagonist RU486 intervention (WA + RU486), corticosterone (CORT) treatment, and CORT treatment plus RU486 intervention (CORT + RU486). Chronic WA stress and CORT treatments significantly down-regulated CLDN1 mRNA levels and concurrent RU486 treatment during the stress phase prevented down-regulation of CLDN1 mRNA levels. Chronic WA stress and CORT treatments significantly down-regulated NR3C1 mRNA levels and concurrent RU486 treatment demonstrated modest preventions. (n = 3, 4; two-tailed test; *P < 0.05; **P < 0.01).

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