Figure 7 | Scientific Reports

Figure 7

From: Chronic stress and intestinal barrier dysfunction: Glucocorticoid receptor and transcription repressor HES1 regulate tight junction protein Claudin-1 promoter

Figure 7

Single-cell resolution quantification of HES1 and NR3C1 proteins in Caco-2/BBe cells before and after 21-day differentiation. Caco-2/BBe cells were fixed at day 1 and day 21 of 21-day differentiation and immuno-stained with HES1 and NR3C1 antibodies for confocal imaging. 100 cells from each group were randomly selected for nucleus immunofluorescence signal quantification. Day 21 cells showed significantly lower nucleus HES1 signal (44.0 ± 0.1%) compared to day 1 cells. Day 1 cells showed only 41.3 ± 0.1% nucleus NR3C1 signal compared to day 21 cells. (n = 100; ***P < 0.001).

Back to article page