Figure 5
Dissection of the BsmR regulon by RNA-seq. (a) bsmR regulon analysis by high-throughput RNA sequencing. The putative target genes were functionally categorized into 10 groups (Supplementary Table S3). (b) qRT-PCR assay shows the positive regulation of fsnR by bsmR. cDNA was reverse-transcribed from the total RNAs of the indicated bacterial strains. The tmRNA level served as the internal control. (c) A biofilm formed by the bacterial strains and stained with crystal violet. (d) Relative quantification of the biofilm levels of the bacterial strains. (e) Swimming motility of the indicated bacterial strains on rich NYG medium plates containing 0.15% agar. (f) Relative quantification of bacterial swimming zone diameters. Each value is the average of three replicates. Error bars represent standard deviation (n ≥ 3). *p < 0.05, as determined by ANOVA. The data are representatives of at least triplicate repeatable experiments.
