Table 1 Strains used in the study.

From: A positive feedback loop mediated by Sigma X enhances expression of the streptococcal regulator ComR

Name

Description

Source

UA159

Wild-type

ATCC 700610

SM061

UA159, but ΔsigX:: kan; KanR

This study

SM065

UA159, but ΔcomS::spc; SpcR (from strain MW02 Mashburn-Warren et al. (2010)

(Khan et al., 2012)

SM066

UA159, but ΔcomR::spc; SpcR (from strain MW05 Mashburn-Warren et al. (2010)

This study

SM068

UA159, p sigX -luc; SpcR

(Khan et al., 2012)

SM089

UA159, but ΔcomS::ery; ErmR

(Khan et al., 2012)

SM091

SM068, but ΔcomS::ery; SpcR ErmR

(Khan et al., 2012)

SM166

UA159, but ΔSMU.60::kan; KanR

This study

SM168

UA159, but ΔSMU.60_c105::kan; KanR

This study

SM170

UA159, but ΔSMU.60_pcomR::kan; KanR

This study

SM180

SM188, but ΔcomS::ery; SpcR ErmR

This study

SM181

SM091, but ΔSMU.60::kan; KanR SpcR ErmR

This study

SM183

SM091, but ΔSMU.60_c105::kan; KanR SpcR ErmR

This study

SM185

SM091, but ΔSMU.60_pcomR::kan; KanR SpcR ErmR

This study

SM188

SM068, but c_105 1-bp substitution; SpcR

(Morrison et al., 2015)

SM218

UA159, but mutated cinbox upstream of c_105; marker-less

This study

SM221

SM091, but mutated cinbox upstream of c_105; SpcR ErmR

This study