Figure 2 | Scientific Reports

Figure 2

From: The Specific Vulnerabilities of Cancer Cells to the Cold Atmospheric Plasma-Stimulated Solutions

Figure 2

The initial several hours after the treatment are key for the anti-cancer effect of PSS. After the treatment for 2 to 4 hr, removing the PSM from cancer cells’ environment would not affect the anti-cancer effect on PA-TU-8988T cells (a) and MDA-MB-231 cells (b). No change means that PSM were used to culture cancer cells for 1 day without renewing the medium. (c) After the treatment for 3 hr, replacing the CAP-stimulated (CAP’s) DMEM by the untreated DMEM just caused a similar anti-cancer effect on PA-TU-8988T cells as using the CAP’s DMEM to affect cells did. (d) The extracellular H2O2 were completely consumed by cancer cells in just 3 hr after the treatment. Results in (a), (b) and (c) are presented as the mean ± s.d. of two independently repeated experiments performed in sextuplicate. Results in (d) are presented as the mean ± s.d. of two independently repeated experiments performed in triplicate.

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