Figure 2

CD34 maintenance but not cell proliferation is influenced by the mode of culture. (A) Comparison of the proportion of CD34⁺ cells after static and dynamic culture over time. HSPCs were cultured under static or dynamic conditions and the percentage of CD34⁺ cells obtained on day 5 and day 9 or day 14 and day 21 was assessed by flow cytometry. Culture mode and day of analysis are given at the bottom. (B) Representative flow cytometry plots of CD34 expression analyses. Day of analysis is given on top, culture mode on the right. White histogram: isotype control; filled histogram: CD34 stained cells. Horizontal line marks gate for CD34⁺ cells. Percentages CD34⁺ cells are indicated at the top left of the respective histograms. (C) Total cell numbers retrieved from the scaffolds after static or dynamic culture on the given day of culture. The table at the bottom gives exact cell numbers ± standard error of the mean (SEM). (D) Numbers of CD34⁺ cells obtained after static or dynamic culture on the given culture day based on the cell numbers and the measured percentages of CD34⁺ cells. Table at the bottom gives exact CD34⁺ cell numbers ± SEM. For d0, d5 and d9: N = 4; for d14 and d21: N = 3 independent experiments. Stat = static culture, dyn = dynamic culture.