Figure 3

Static culture preserves a greater pool of CD34^high cells compared to dynamic culture in the early phase of culture. Comparison of percentages of CD34^high and CD34^low cells obtained from static and dynamic culture on day 5 and day 9. (A) Representative flow cytometry plots of CD34 expression analysis. Mode of culture is given on the right, the gates determining CD34^low and CD34^high cells are given on top. (B) Percentages of CD34^high and (C) CD34^low cells as assessed by flow cytometry. (D) Stacked column chart showing the percentage of CD34⁺ cells within the harvested cells subdivided into the percentages of CD34^low and CD34^high cells. White bars give the percentage of CD34^low cells, striped bars the percentage of CD34^high cells. (A–D) N = 4 independent experiments.