Figure 4
From: 3D models of the hematopoietic stem cell niche under steady-state and active conditions
Impact of static and dynamic culture on HSPC differentiation capacity. (A,B) CFU assay of 500 cells directly after HSPC isolation from UCB (d0) or after 5, 9, 14 or 21 days of static or dynamic culture. (A) Summary of generated colony types and frequencies for each sample. Significance was tested based on total number of produced colonies. (B) Overview of generated colonies for each colony type separately (C) Percentages of cells expressing either CD2, CD14, CD19 or CD71 (markers for T-cells, myeloid cells, B cells and megakaryocyte/erythrocytes, respectively) as assessed by flow cytometric analysis of HSPCs after 9 days of static or dynamic culture. (A,B) Day 0, day 5 and day 9: N = 4 independent experiments; day 14 and day 21: N = 3 independent experiments; (C) N = 3 independent experiments, significance was tested with paired, two-tailed student’s t-test.
