Figure 6
From: 3D models of the hematopoietic stem cell niche under steady-state and active conditions
The culture mode affects the sensitivity of HSPCs to the myelotoxic drug 5-fluorouracil. (A) Schematic representation of the experiment. HSPCs were cultured together with MSCs in standard 2D tissue culture plates or in macroporous 3D scaffolds under static or dynamic conditions in the presence of 5-fluorouracil (5FU) or DMSO as a control for 5 days. Cells are drawn in violet, the scaffold is represented in blue, secreted molecules in green and fluid flow is indicated by black arrows. (B) The response of HSPCs to the 5FU treatment by cell death was assessed by Sytox staining and the results are presented as circle diagrams. Percentage of CD34+ cells is depicted in blue, percentage of CD34− in orange, live (Sytox−) cells in light color and dead or late apoptotic cells (Sytox+) in dark blue or dark orange. (C) The percentage of Sytox+ cells within the CD34+ (blue) and CD34− subpopulation (orange) of cells after 5 days in the presence of 5FU under the different culture conditions are presented as bar graph with error bars (mean ± standard deviation). N = 3 independent experiments.
