Figure 3 | Scientific Reports

Figure 3

From: Zn(II) mediates vancomycin polymerization and potentiates its antibiotic activity against resistant bacteria

Figure 3

Interaction of Zn(II) with vancomycin increases its activity against glycopeptide-resistant peptidoglycan precursors terminating in D-Ala-D-Lac, and changes its binding. (a) The effect of Zn(II) on the activity of teicoplanin (T), vancomycin (V), bacitracin (B) and kanamycin (K) against S. coelicolor strains engineered to constitutively produce peptidoglycan precursors terminating in D-Ala-D-Lac (H7400) or D-Ala-D-Ala (H7300). Supplementation with D-lactate (D-Lac; 10 mM) promotes the major D-Ala-D-Lac ligase activity of VanA at the expense of the minor D-Ala-D-Ala activity. (b) Fluorometric analysis indicating a change in the interaction between vancomycin and D-Ala-D-Lac, but not D-Ala-D-Ala, in the presence of Zn(II). The left panel shows the effect of Zn(II) on vancomycin fluorescence for comparison.

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