Figure 5 | Scientific Reports

Figure 5

From: The Enterococcus faecalis virulence factor ElrA interacts with the human Four-and-a-Half LIM Domains Protein 2

Figure 5The alternative text for this image may have been generated using AI.

Cellular localisation of untruncated and FID-truncated forms of ElrA and FHL2. HA-FHL2 and myc-ElrA were produced after transfection of HeLa cells with the pCMVHA-FHL2 and pCMVmyc-ElrA27–723 or pCMVmyc-ElrA27–473 plasmids. A representative cell is shown for each form of myc-ElrA. In the left panels (A and D), is represented the localisation of the untruncated (A) and the FID-truncated ElrA (D) the FHL2 staining (anti-HA) is represented in the middle panels (B and E), and the right panels were generated by merging the anti-myc and anti-HA signals (C and F). Scale bars = 10 µm. Localization of HA-tagged FHL2 (B) is similar (C) to the localization of an untruncated myc-ElrA (A) whereas localization of HA-tagged FHL2 (E) is different (F) to the localization of a truncated myc-ElrA (D).

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