Figure 4
Serum withdrawal does not modulate the effect of hyperosmolarity on autophagy in NP cells. (a) Western blot analysis of NP cells cultured in serum-free media with increasing osmolarity (330–600 mOsm/kg H2O) showed that the levels of autophagic markers including LC3-II, ATG12-ATG5, and BECN1 were unaltered. SQSTM1 levels did not change with increasing osmolarity except for a small decrease at 600 mOsm/kg H2O. (b,c) Densitometric analyses of multiple Western blots. Bars represent mean ± SEM (n = 5). One-way ANOVA with Sidak’s multiple comparisons test was used to determine statistical significance. NS, non-significant. Western blot images were cropped and acquired under same experimental conditions. See Supplementary Fig. S1 for examples of uncropped images.
