Figure 4 | Scientific Reports

Figure 4

From: Surface modification of pig endothelial cells with a branched heparin conjugate improves their compatibility with human blood

Figure 4

CHC coating of microbead-borne PAEC inhibits formation of soluble complement, coagulation and fibrinolytic markers during incubation with human blood. EDTA- and citrate-plasma samples were collected from the whole blood coagulation assays at 20 min and assayed by ELISA for (A) C5a, (B) sC5b-9, (C) PAI-1/tPA complex in EDTA-plasma and (D) TAT complex and (E) D-dimers in citrate plasma. Coating of WT PAEC with CHC significantly reduced the plasma levels of all 5 markers. GTKO.hCD46.hTBM PAEC (no CHC) induced significantly lower levels of the markers than WT PAEC (no CHC); CHC coating of the GTKO.hCD46.hTBM PAEC further reduced TAT complex and D-dimers formation. Data are mean ± SD of four independent experiments. Significance was tested using one-way ANOVA with Bonferroni correction (*p < 0.05, **p < 0.01, ***p < 0.001).

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