Figure 2 | Scientific Reports

Figure 2

From: MicroRNA profiling provides insights into post-transcriptional regulation of gene expression in chickpea root apex under salinity and water deficiency

Figure 2

Expression analysis of genome-annotated conserved miRNAs in chickpea root apex under PEG and salt treatment. (A) Northern blot was performed to analyse expression levels of miRNAs at 2 mm region of chickpea root apex after 1 hr and 2 hrs of PEG and salt treatments. 15 µg of enriched small RNA from control and treated samples was loaded on denaturing (7 M urea) polyacrylamide (15%) gel. Radiolabeled antisense probes were used for hybridization. Ethidium bromide-stained small RNAs were shown for equal loading. SnoRD24 was used as control. (B) Expression pattern of the miRNAs shown in Fig. 2A and their predicted target genes in response to the same treatments as assessed by qRT-PCR. Standard deviations of three replicates were shown. CaEF1α was used as internal control for normalization. Grey and black lines represent salt and water deficit stress treatments, respectively. While dotted and solid lines represent miRNA and target gene, respectively.

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