Figure 4
From: Naringenin Impairs Two-Pore Channel 2 Activity And Inhibits VEGF-Induced Angiogenesis
Naringenin impairs VEGF-induced vessel formation in vitro. (a) Representative images of one of three independent experiments. HUVECs were plated in Matrigel-coated dishes and incubated for 3–4 h in EBM-2 + 2% FBS supplemented or not with VEGF or Nar, or in medium containing both VEGF and Nar. (c) Representative images of one of three independent experiments. HUVECs were plated in Matrigel-coated dishes and incubated for 2–3 h in EGM-2 supplemented or not with Ang-1 or Nar, or in medium containing both Ang-1 and Nar. (b,d) Quantitative evaluation of tube formation as the number of closed polygons formed in 6 fields for each experimental condition for VEGF (b) and Ang-1 (d). Data in bar charts represent mean ± s.e.m. of three independent experiments. *P < 0.05.
