Figure 5
From: Probing the missing mature β-cell proteomic landscape in differentiating patient iPSC-derived cells
Comparative IPA analyses of the human islets and S7 cell population. (a) Experimental design depicting the IPA analysis of proteins that were identified exclusively in human islet-specific and S7-cells molecular, respectively. The data represents individual samples from the 10-plex. The proteome of in vitro generated S7 cells showed 1874 (yellow pie) uniquely identified proteins as compared to 996 (orange pie) uniquely identified in human islets. (b) The top 5 upstream regulators predicted from the human islet-specific molecular landscape. (c) The top 5 upstream regulators predicted from the S7-cell specific molecular landscape. (d) Experimental design depicting the analysis of the differentially expressed between S7 cell proteome compared and human islets. Note that samples were combined before they were run enabling relative quantification. (e) The top 5 upstream regulators predicted from the proteins differentially expressed between S7 cells and human islets. (f) PCP pathway components predicted as activated by IPA from the proteins differentially expressed between S7 cells and human islets.
