Figure 2
From: Revealing enterovirus infection in chronic human disorders: An integrated diagnostic approach
Results of end-point PCR of quantitated pU57 plasmids containing the 5′UTR region of CV-A6, CV-B3, PV-1, EV-D68 (representative enteroviruses of the A, B, C, and D species). Ten-fold dilutions of each plasmid were tested by end-point PCR with two different primer pairs: 5′UTR-Tok (panel a) and 5′UTR-C (panel b). For each gel, six lanes of cropped gels are shown (left to right): DNA size markers, PCR amplicons obtained with 1.6, 16, 160, 1,600, or 16,000 template molecules per reaction. Primer pairs were detecting ≤16 template molecules/reaction. Original, uncropped images are presented in the Supplementary Materials (Figures S1 and S2).
