Table 5 Sequence of enterovirus amplicons obtained from cases of post-polio syndrome (PPS), type 1 diabetes (T1D), and chronic viral cardiomyopathy (CVC).
From: Revealing enterovirus infection in chronic human disorders: An integrated diagnostic approach
Diagnosis | Case | Genome Region | Amplicon Sequence | Closest match in BLAST | |||
|---|---|---|---|---|---|---|---|
Accession No. | Description | Identities, gaps | EV species | ||||
PPS | RBJ/va15 | 5′UTR | GGCTAATCCTAACGATGGAGCACGCAGCTGCAACCCTGCAGCCAACCTGTCTTAACGCGCAAGTCCGTGGCGGAACCGACTACTTTGGGTGTCCGTGTTTCCTTTTATTCTTGAATGGCTGCTTATGTGACAATCACAATCA | KX162706.1 | Human poliovirus 2 strain NIE0611579 | 131/137, 1 | C |
5′UTR | TGGTGTTGGTGTGAAGAGCATATTGAGCTACATGAGAGTCCTCCGGCCCCTGAATGCGGCTAATCCTAACGATGGAGCACGCAGCTGCAACCCTGCAGCCAACCTGTCTTAACGCGCAAGTCCGTGGCGGAACCGACTACTTTGGGTGTCCGTGTTTCCTTTTATTCTTGAATGGCTGCTTATGTGACAATCACAATCA | KU763188 | Human poliovirus 3 isolate 45507, Sabin-derived | 180/187, 1 | C | ||
LZL/va10 | 5′UTR | TCATGAGATCCTCCGGCCCCTGATGCGGCTAATCCTAACCATGGAGCAGGTAATCGCAAACCCAGCGGTCAGCCTGTCGTAACGCGTAAGTCTGTGGCGGAACCGACTACTTTGGGTGTCCGTGTTTCCTTTTATTTTTATGGGGGTGGTTAAGGGGGCCATCCAGA | KP793687.1 | Human poliovirus 1 strain Brunenders | 151/163, 4 | C | |
CCL/va12 | 5′UTR | GCTGCTTTATGGTGACAATCAGAGATTGTTATCATAAAGCGATTTGGATTGGCCATCCGGTGTGTGTTGCATCAAATACGTTAATACTTGTTTAAACTATTGTATTAATTTTACCCTTCTCTTAATCAATCACTCATAAACACTACGAGGATTGAATTACAGT | DQ890387.1 | Human poliovirus 2 strain USA9810768, Sabin-derived from chronic immunodefficient patient | 132/163, 5 | C | |
5′UTR-VP2 | GCTGCTTTATGGTGACAATCAGAGATTGTTATCATAAAGCGATTTGGATTGGCCATCCGGTGTGTGTTGCATCAAATACGTTAATACTTGTTTAAACTATTGTATTAATTTTACCCTTCTCTTAATCAATCACTCATAAACACTACGAGGATTGAATTACAGTACTACAATGGGAGCCCAAGTGTCGACACAGAAAGTCGGAGCTCACGAAAATTCAAATAGAGCCTATGGCGGGTCCACCATCAATTACACTACAATCAATTATTACAGAGATTCTGCAAGCAGTGCTGCGAGCAAGCAAGATTTTGCTCAAGACCCATCCAAGTTCACTGAGCCCATCAAGGATGTCCTCATAAAGACCGCACCCATGCTGAACTCCCCGAACATTGAGGCGTGTGGTTACAGTGATAGAGTAATGCAATTAACTCTGGGTAACTCAACAATCACCACTCAAGAGGCGGCCAACTCCGTTGTTGCTTACGGCAGATGGCCTGAATACATCAGAGATTCTGAGGCAAATCCCGTGGACCAACCAACCGAACCCGACGTGGCTGCGGTGCAGGTTTTACACGTTGGACGCCCAGAGCTTTGAATAAAGCTGGGAT | 495/579, 6 | C | ||||
T1D | MZA/va15 | 5′UTR | GTTGGCGGCCAGCCCACTGGGGCAACCCATGGGACGCTTCAATACTGACATGGTGCGAAGAGTCTATTGAGCTAATTGGTAGTCCTCCGGCCCCTGAATGCGGCTAATCCTAACTGCGGAGCAGGCACTCGCAGACCAGCGAGCAGCTTGTCGTAATGGGCAACTCCGCAGCGGAACCGACTACTTTGGGTGTCCGTGTTTCCTATTTCCTTTATATTGGCTGCTTATGGTGACATATAAGGAAA | AF114384.1 | Coxsackievirus B6 strain Schmitt | 231/235, 1 | B |
5′UTR | TGGCTGCGTTGGCGGCCAGCCCACTGGGGCAACCCATGGGACGCTTCAATACTGACATGGTGCGAAGAGTCTATTGAGCTAATTGGTAGTCCTCCGGCCCCTGAATGCGGCTAATCCTAACTGCGGAGCAGGCACTCGCAGACCAGCGAGCAGCTTGTCGTAATGGGCAACTCCGCAGCGGAACCGACTACTTTGGGTGTCCGTGTTTCCTATTTCCTTTATATTGGCTGCTTATGGTGACA | 238/242, 1 | B | ||||
FDF/va15 | 5′UTR | GTCCTCCGGCCCCTGAATGCGGCTAATCCTAACTGTGGAGCAGGATAGCCTGACAGACACCGCTGGCCCAGCCCTGTCGTAACGGGCAAGCTCTGTCGAGCGGAACCGACTACTTTGGTGACCGTGTTTCA | JX898908.1 | Coxsackievirus B1 strain Groningen/2011 | 112/131, 11 | B | |
NPD/va14 | 5′UTR | ATGTTGCCATATAGCGATTGGCTTGGCCTCCGGTGTCCAATAAAGCGATCATTTATTTGTTTGTTGGGTTCGTACCCTTGAATTACAAAGCAATTGTAACGCTTACCTAACTGCTGTGCCACTGAATTCAGATCTCCCGGGCCA | AM237001.1 | Human echovirus 30 isolate CF2249-01 | 95/105, 1 | B | |
5′UTR-VP2 | CTACTGCGGACAGAACCTACACGCCAGTGGGCAGTCTGTCGTAACGGGCAACTCCGCAGCGGAACCGACTACTTTGGGTGTCCGTGTTTCCTTTTTCTTTATACTGGTTGCTTATGGTGACAATTGAGAGATTGTTGCCATATAGCTATTGGCTTGGCCATCCGGTGTCCAATAAAGCGATCATTTATTTGTTTGTTGGGTTCGTACCCTTGAATTACAAAGCAATTGTAACGCTTAAATATATTATAGACCTCAACACAGCAAAATGGGGGCCCAAGTTTCAACACAAAAAACTGGAGCTCATGAGACCGGCTTGAGTGCCAGTGGAAACTCCATTATACATTACACAAATATTAATTACTACAAGGACTCTGCTTCTAACTCATTGAGCCGGCAAGACTTTACCCAAGATCCCAGTAAATTCACAGAACCGGTGAAGGATGTGATGATTAAGACCTTGCCCGCTTTAAATTCACCCACTGTAGAAGAATGCGGCTTCAGTGACCGAGTGCGGTCGATCACCCTGGGGAATTCTACGATCACCACACAGGAGTGTGCTAACGTGGTTGTGGGATAC | Human echovirus 30 isolate CF1570-02 | 550/578, 3 | B | |||
CVC | BZS/va13 | 5′UTR | GCCTCCGGCTCCTTAAGGTGACTAATCCCTAACTGCGGAGCACACACCCTCAAGCCAGAGGGCAGTGTGTCGTAACGGGCAACTCTGCAGCGGAACCGACTACTTTGGGTGTCCGTGTTTCATTTTATTCCTATACTGGCTGCTTATGGTGACAATCACCC | KF986401.1 | Human coxsackievirus B3 strain MCCV1S | 149/155, 1 | B |
PLF/va12 | 5′UTR | TGGTTGGTGGGCCTCCGGCTCCTTAAGGTGACTAATCCCTAACTGCGGAGCACACACCCTCAAGCCAGAGGGCAGTGTGTCGTAACGGGCAACTCTGCAGCGGAACCGACTACTTTGGGTGTCCGTGTTTCATTTTATTCCTATACTGGCTGCTTATGGTGACAATTTGAGAGATTGTTCCATATAGCTATTGGATTGGCCACTACTTGATGCCCCGGGAGCTATTATATATCTCTTTGTTGGGTTTATACCACTTAGCTTGAAAGAGGTTAAAACATTACAATTCATTGTTAAGTTGAATACAGCAAAATGGGAGCTCAAGTATCAACGCAAAAGACTGG | KJ025083.1 | Human coxsackievirus B3 strain MKP | 319/340, 5 | B | |
5′UTR-VP4 | TGGTTGGTGGGCCTCCGGCTCCTTAAGGTGACTAATCCCTAACTGCGGAGCACACACCCTCAAGCCAGAGGGCAGTGTGTCGTAACGGGCAACTCTGCAGCGGAACCGACTACTTTGGGTGTCCGTGTTTCATTTTATTCCTATACTGGCTGCTTATGGTGACAATTTGAGAGATTGTTCCATATAGCTATTGGATTGGCCACTACTTGATGCCCCGGGAGCTATTATATATCTCTTTGTTGGGTTTATACCACTTAGCTTGAAAGAGGTTAAAACATTACAATTCATTGTTAAGTTGAATACAGCAAAATGGGAGCTCAAGTATCAACGCAAAAGACTGGGGCACATGAGACCGGGCTGAATGCTAGCGGCAATTCCATCATTCACTACACAAATATTAATTATTACAAGGATTAGCCACATT | 392/413, 5 | B | ||||
LZS/va09 | 3Dpol | TCTTCTATCCCTGGCCTATAAACTCTTCCCCGTTGGCGCACATTGGTAAGGGTTACGGATTGATCATGACACCAGCTGATAAAGGAGAGTGTTTCAATGAAGTCACCTGGACAAACGTCACCTTCCTGAAAAGGTATTTCAGAGCAGATGAGCAATATCCATTCCTAGTACATCCAGTCATGCCCATGAAAGATATACATGTTTCTATTCGATGGACTTATGACCCAC | U05852.1 | Echovirus 6 non-lytic persistent strain | 189/227, 1 | B | |
5′UTR | ACCAATTACTTCGAGATTCCTAGTATCACCATCAAGGTTGGGCAGTGTTACCCTCCGCACAACTTCAGTGTAGATCAGGCCGATGAAGCACCGCACTCCGTACGGGCGACCGTGGCGGTGCCTGCGCTGGCGGCTAGCCCATATCCCAACCCATGGGCCGCTTCAAAACTGACTTGGTCTGAACTGTCTATTGAGCTAATTGGTAGTTATCCGGCCCCTGAATGGGCCTAATCCTAACTGCGCCGCAGATACCTACATGCCAGTCTGCGGAGTCTGTCGTAACGGGCAACTCCGCGGCGGAATCGACTACTTTGGGTGTGGGTGTTTCCTGTTATTTTATTCTGGGTTGTTATTGGGACCATTGAGAGATTGTTATCA | AB705311.1 | Echovirus 6 strain Hokkaido/19724/2011 | 330/379, 3 | B | ||
