Figure 3 | Scientific Reports

Figure 3

From: Retinoic Acid affects Lung Adenocarcinoma growth by inducing differentiation via GATA6 activation and EGFR and Wnt inhibition

Figure 3

RA affects EGFR signaling activation in TKI resistant NSCLC cell line. (a) Representative Western Blotting analysis of EGFR and pEGFR in H-1975 and HCC827R1 cells treated with 1 and 10 µM RA for 48 and 72 hrs (n = 3). (b) Densitometric analysis of EGFR and pEGFR protein levels in H-1975 (left panel) and HCC827R1 (right panel) NSCLC cell lines upon RA treatment for 48 and 72 hrs. The analysis has been performed by using GAPDH as normalizer loading control (n = 3) (*=0.02). (c) qRT-PCR analysis of EGFR H-1975 NSCLC cells treated with 1 and 10 µM RA for 24, 48 and 72 hrs. The comparison has been conducted by using the ΔΔCT method and normalized to GAPDH transcript. Dotted line represents the normalized expression levels of each transcript analyzed in untreated cells. Data are represented as mean ± SD. Statistical analysis has been obtained by unpaired t test (n = 3) (*=0.02). (d) Representative FACS plot representing AKT and pAKT levels in H-1975 cells treated with RA for 72 hrs (n = 3). (e) Quantification analysis of pAKT levels in H-1975 cells treated with RA for 48 and 72 hrs (n = 3) (*=0.02).

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