Figure 5
Identification of bacteria in a pure culture. (a–e) Data obtained from the photodiode array designed by scanning LB agar either sterile or with an inoculation of E. coli or S. aureus. Incident light angle was changed for each of the five data sets; 135° (a), 120° (b), 110° (c), 100° (d), and 90° (e). At each individual incident light angle, significant differences result between bacterial inoculation and control samples as well as between each species of inoculation; E. coli or S. aureus. As expected, the trend changes as the incident light angle changes, but distinct differences are maintained despite changing trends with changing incident angles. One data point is considered to be the average of 12 readings from each photodiode (3 s scan). Each of five agar samples was scanned at three unique locations for 3 s at each location. Error bars represent standard error of the mean. * is shown to the left side of each data point indicating significant difference (p < 0.05) between E. coli vs. control samples or between S. aureus vs. control samples. † is shown to the right side of each data point indicating significant difference (p < 0.05) between E. coli vs. S. aureus samples.
