Figure 6

The lack of α2-Na⁺,K⁺-ATPase decreases TNF-α levels. Cell culture supernatants of scrambled sequence and siRNA cells after treatment with LPS (1 µg/mL) and OUA (10 µM) were collected and used to measure TNF-α levels by ELISA (n = 6). (A) The levels of TNF-α in the scrambled group, *p < 0.05 vs control and OUA. (B) The levels of TNF-α in the siRNA group, *p < 0.05 vs control and OUA. (C) Comparison of the levels of TNF-α in the siRNA and scramble groups, *p < 0.05 vs scramble + LPS and scramble + O + L.