Figure 1

Morphological and self-renewal evaluation by clonal analysis. (a) Representative flow cytometric profiles of rBM cells stained for CD29 and CD54. PI⁻, CD45⁻, and CD31⁻ cells were gated. Baseline CD29 and CD54 expression is shown in pink. Sorted subsets are shown in black dotted squares. The cell number ratio is shown in the profile. (b) Morphological appearance of sorted sub-population cells. Scale bar, 100 µm. (c) Single-cell sorting assay of rBM cell sub-populations. WBM cells and four cell populations (CD29⁺/CD54⁺, CD29⁺/CD54⁻, CD29⁻/CD54⁺, and CD29⁻/CD54⁻) were sorted into 96-well plates, and the number of colonies formed was counted on day 10. Scale bar, 500 µm.