Figure 2 | Scientific Reports

Figure 2

From: Potassium as a pluripotency-associated element identified through inorganic element profiling in human pluripotent stem cells

Figure 2The alternative text for this image may have been generated using AI.

Alteration in NANOG and POU5F1 protein expression in WA09 hESCs and HDF51i-509 hiPSCs treated with potassium channel modulators and a potassium ionophore. (A) Potassium channel blockers (tetraethylammonium and 4-aminopyridine) induced the dose- and time-dependent downregulation of NANOG and POU5F1 detected by Western blotting. (B) A potassium ionophore, salinomycin, caused a dose- and time-dependent upregulation of NANOG and POU5F1. NANOG was upregulated by a potassium channel activator, diazoxide, in both hESCs and hiPSCs, while POU5F1 appeared to be relatively unaffected. (C) The chemical structures of the potassium channel modulators and ionophore. (D) The expression and salinomycin-induced upregulation of NANOG and POU5F1 in WA09 hESCs were attenuated by adding 60 mM KCl in the culture medium. (E) The 36-hour treatment of TEA promotes the downregulation of NANOG and POU5F1 in WA09 hESC aggregates cultured in the FGF-deficient medium at the beginning of EB formation. Normalized NANOG and POU5F1 protein band intensity from densitometry analysis was shown at the bottom of each panel of Western blotting images. Representative blot images displayed in this figure were cropped from original blots shown in the Supplementary Information and organized into composite panels.

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