Figure 6
MALDI-TOF MS analysis of CHOS/TG products with DP5 and DP6 substrates catalyzed by EcChi1. The each 40 μL of reaction sample was concentrated under reduced pressure at 25 °C and dissolved in 10 μL of MilliQ H2O. The 2 μL of the reaction mixture was mixed with equal volume of 2,5-dihydroxybenzoic acid (2,5-DHB), and the resultant solution was subjected to mass measurements using an Ultraflex MALDI – TOF/TOF instrument. The masses shown here are with Na adducts.
