Figure 6 | Scientific Reports

Figure 6

From: Hyperosmolarity-induced AQP5 upregulation promotes inflammation and cell death via JNK1/2 Activation in human corneal epithelial cells

Figure 6

Effect of AQP5 gene silencing on hyperosmolarity-induced rises in proinflammatory cytokine expression levels. After AQP5 gene expression was silenced by targeted siRNA, the immortalized HCEC were cultured in 500 mOsm medium for 4 h (for RT-PCR) and 24 h (for western-blot). The mRNA levels of AQP5, IL-1β, IL-6, IL-8, TNF-α, and caspase-1 were significantly inhibited in the siAQP5 group compared with the siNC group (P ≤ 0.039) (A). Inhibition of AQP5 protein expression in AQP5 gene silenced cells confirms the targeted gene was efficiently knocked down (B). The cropped gels are displayed and the full-length gels are provided in a Supplementary Information file. * Indicates P < 0.05 in the comparsion between the 500 mOsm group and 310 mOsm group; and ** indicates P < 0.05 in the comparsion between the 500 mOsm + siAQP5 group and the 500 mOsm + siNC group.Three independent experiments were conducted with 3 repeats each.

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