Figure 1
From: “High-Throughput Characterization of Region-Specific Mitochondrial Function and Morphology”
Algorithm to align biosorter data and derive positional information. (A) Representative profile for a single nematode displaying longitudinal information in: Extinction (Black) and tissue-specific fluorescent signals (Green = Neurons, Red = Intestine). Anterior is to the left. “A.U.” = Arbitrary Units. (B,C,E,F) Illustration of orientation algorithm devised to align animals. Example shows individual profiles (Y axis) represented as in (A) with signal intensity plotted as a function of the animal’s length (X axis). Tissues have been labeled (red in cartoons above) to enable orientation of nematodes (B). Data is shown in a heat map with Red as the most signal and Blue as the least. (D) Demonstration of an average profile algorithm following alignment. (Top) Representative micrographs of transgenic worms analyzed below with intestine (Red, vha-6p ::mCherry::HDEL) and sensory neurons (DiO, Green) fluorescently labeled. Worms are oriented with their anterior to the left. Scale = 200 µm. (Bottom) Median profile from 100+ animals in the Red (intestine) and Green (neurons) channels. Data is shown in a heat map with Red as the highest intensity signal and Blue, the lowest. All animals are positioned with the anterior side to the left. Plots are representative of three biological replicates and derived from n ≥ 50 organisms. Sample size (n) for all experiments can be found in the Supplementary Table. L.A. Daniele provided the illustrations.
